A big day of updates for SRA737
On 27 February 2018, Sierra provided a comprehensive update on its clinical programs for the development of SRA737. SRA737 is an inhibitor of Chk1, a key protein for the regulation of the replication stress response (for a useful review of the replication stress response and cancer, see Forment & O’Connor 2018).
Initial safety data were provided for both the SRA737 monotherapy and gemcitabine combination studies. The company has decided to expand both the number of indications being examined and the number of patients per cohort for both of these studies, increasing the combined enrolment in the Phase II portions to 200. It announced that the SRA737 monotherapy study has already progressed to the Phase II dose-expansion portion of the trial, which is enrolling well across the genetic subtypes being examined, and that it intends to enter the Phase II portion of the combination study in Q218.
The company also provided a rationale for a study examining SRA737 in combination with the poly ADP-ribose polymerase (PARP) inhibitor, Zejula (Janssen/Tesaro). The company concurrently announced that it had reached an agreement with Janssen to provide the drug for a Phase Ib/II study in patients with metastatic castration-resistant prostate cancer (mCRPC). The study is expected to initiate in Q418. In other news, the company provided an up-to-date timeline for the initiation of an SRA737/immuno-oncology combination study for which it expects to have clinical trial authorization in Q418. Finally, it announced that its Cdc7 inhibitor SRA141 should have a completed IND application submitted in H218.
SRA737 monotherapy dose-escalation complete
In February 2018, the company completed the dose-escalation portion of its ongoing SRA737 monotherapy trial after enrolling 11 patients and proceeded to the expansion portion of the trial (20 patients enrolled at the time of the update). The Phase I/II clinical trial was designed with two phases:
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A Phase I ascending dose portion. Patients were all comers and not preselected based on genetic criteria. Readouts are maximum tolerated dose and safety.
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A Phase II dose-expansion cohort in patients with mutations potentially conferring sensitivity to SRA737. Readouts are efficacy and safety.
The dose escalation identified 1,000mg a day as the maximum tolerated dose, following two dose-limiting toxicities (DLTs) experienced at the 1,300mg dose. Per the protocol, a DLT is defined as events preventing the patient from receiving at 75% of the planned dose, in this case triggered by two cases of GI intolerability. The company is also currently investigating 500mg twice a day as an alternative to the single daily dose.
The adverse event (AE) profile observed in the trial (from all 31 patients to date) is described in Exhibit 1. The most common AEs were fatigue and GI related (over 20% of events combined), and a majority of events were Grade 1 or 2. Neutropenia and other hematologic toxicities were also observed in the trial and, although most cases were Grade 1 or 2, there were two Grade 3 reports at high doses, one of which was a serious adverse event (SAE).
Exhibit 1: SRA737 adverse event profile
Adverse events |
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Hematologic |
Grade 3 neutropenia in two patients (7%) at 1,300mg and 1,000mg |
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One grade 3 was an SAE |
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<20% with grade 1, 2 |
GI |
Two GI related DLTs (inability to receive 75% of planned dose) at 1300mg QD |
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Grade 3 nausea in two patients (7%) |
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Remaining cases primarily Grade 1 |
Cardiac |
One Grade 3 heart failure/cardiomyopathy (SAE) |
Adverse events |
Hematologic |
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GI |
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Cardiac |
Notes |
Grade 3 neutropenia in two patients (7%) at 1,300mg and 1,000mg |
One grade 3 was an SAE |
<20% with grade 1, 2 |
Two GI related DLTs (inability to receive 75% of planned dose) at 1300mg QD |
Grade 3 nausea in two patients (7%) |
Remaining cases primarily Grade 1 |
One Grade 3 heart failure/cardiomyopathy (SAE) |
Both GI and hematologic AEs are consistent with the mechanism of action of the drug, and are common for this class. Previous reports of AEs for the Chk1 inhibitor prexasertib (Eli Lilly) also showed these events. Although the results are not strictly comparable due to different trial structures, in one recent study prexasertib showed significantly more AE of these types in Phase II: 64% of patients (out of 28) experienced Grade 1 nausea and 93% of patients experienced neutropenia, 79% at Grade 4. The AE profile of Genentech’s second-generation Chk1 also shows more common events: 80% neutropenia and 46% nausea as the most common of these two classes (all grades).
There was one other SAE: a Grade 3 heart failure in a patient in the 1000mg cohort. The company (as well as invited speakers) noted that this patient had rapidly progressing disease, which could contribute to cardiac events, although it cannot be ruled out as a drug effect. Dose-limiting heart failure (as well as a dose-limiting troponin increase) was seen in clinical trials of the first-generation Chk1 inhibitor AZD7762 (AstraZeneca) However, these AEs were considered off target effects and heart failure is not a feature of this class of drug (although MK-8776 from Merck did show dose-limiting QT prolongation). Due to its isolated nature, we do not consider this event to be of particular concern at this point.
Phase II enrolling well across tumor genetics
The company has already begun enrolling patients into the Phase II expansion portion of the monotherapy trial at three pilot sites. This portion of the trial, unlike the Phase I portion, will only enrol patients with certain predefined genetic tumor backgrounds expected to respond to SRA737. A potential concern is that, given the many different combinations of mutations and tumor types, the expected prevalence of these different subgroups would not be born out in the clinic, and there would be gaps in the data set. A host of clinical factors could contribute to these issues, if for instance doctors favoured certain therapies over this clinical trial for a particular group or if the prognosis for a particular group was too poor to enable enrolment.
At the investor update, the company reported that patients are being enrolled in accordance with the underlying rates of these tumor mutations. Although only 20 patients have been enrolled, eight of the 16 highlighted subgroups have enrolled at least one patient (Exhibits 2 and 3). The company announced that it is expanding the cohort size for each cancer type to 20 patients (from eight), and we believe that with these encouraging early data, it should provide sufficient coverage of the expected genotypes.
Exhibit 2: Expected prevalence of subgroups
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Exhibit 3: Real enrolment of subgroups
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Source: Sierra Oncology. Note: HNSCC = head and neck squamous cell carcinoma. HGSOC = high-grade serous ovarian cancer. mCRPC = metastatic castration-resistant prostate cancer. CRC = colorectal cancer. NSCLC = non-small cell lung cancer.
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Exhibit 2: Expected prevalence of subgroups
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Exhibit 3: Real enrolment of subgroups
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Source: Sierra Oncology. Note: HNSCC = head and neck squamous cell carcinoma. HGSOC = high-grade serous ovarian cancer. mCRPC = metastatic castration-resistant prostate cancer. CRC = colorectal cancer. NSCLC = non-small cell lung cancer.
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One factor that the company has highlighted in this release is the inclusion of mutations along the CCNE1 axis as a genetic subgroup it is monitoring, although only two patients have been enrolled in this set to date. CCNE1 is the gene expressing cyclin E1, a protein important for cell cycle progression (similar to Chk1) and a known tumor driver. In Q118, the company amended the trial protocol to include CCNE1-driven, high-grade serous ovarian cancer (HGSOC) as a new cohort (in addition to the previous platinum-resistant ovarian cancer cohort), bringing the expected total enrolment for the trial to 120 patients. There is increasing evidence of CCNE1 as a tumor driver that induces replication stress. Recent Phase II results of prexasertib in ovarian cancer identified a higher response rate in patients with a mutation or amplification this gene. The company additionally presented its own preclinical data supporting the activity of SRA737 in this subgroup (Exhibits 4 and 5). The company will be presenting additional information on the activity of SRA737 in CCNE1 driven ovarian cancer at the American Association of Cancer Research (AACR) meeting in April 2018.
Exhibit 4: Patient derived xenograft model of HGSOC
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Exhibit 5: OVCAR3 cell line model of HGSOC
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Exhibit 4: Patient derived xenograft model of HGSOC
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Exhibit 5: OVCAR3 cell line model of HGSOC
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The company has guided to preliminary data being available from the trial at a medical conference in Q418.
Low-dose gemcitabine trial update
The company is still in the dose-escalation portion of its Phase I/II trial of SRA737 in combination with low-dose gemcitabine. The trial is examining a range of doses for each drug to find the optimal combination (Exhibit 6). Gemcitabine is an inducer of replication stress and can directly potentiate the clinical activity of SRA737, even at low concentrations. The company is therefore examining concentrations of gemcitabine that are dramatically lower than cytotoxic levels (Exhibit 7).
Exhibit 6: Doses of SRA737 and gemcitabine in dosing cohorts
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Exhibit 7: Gemcitabine dose ranges
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Exhibit 6: Doses of SRA737 and gemcitabine in dosing cohorts
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Exhibit 7: Gemcitabine dose ranges
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The company provided some preliminary safety data from the combination study, which were also in line with expectations. GI and hematological AEs dominated, but were mostly Grade 1 and 2. One Grade 3 neutropenia was seen with the highest dose of gemcitabine (40mg SRA737, 300mg gemcitabine). Two SAEs were observed: a Grade 1 fever and a Grade 2 deep vein thrombosis, which we do not consider to be of particular concern.
Similar to the monotherapy trial, the company is expanding the number of indications and the number of patients it is enrolling in each cohort for this study. The upcoming Phase II portion of the trial will enrol patients with small cell lung cancer, sarcoma and cervical/anogenital cancer in addition to the previous indication of bladder cancer. Pancreatic cancer has been removed from the protocol as a target indication. Additionally, each of these cohorts will now enrol a target of 20 patients. The Phase II cohort expansion potion of the trial is expected to start in Q218, and the company has stated that it anticipates providing an update in Q418.
Janssen to provide Zejula for PARP inhibitor combo study
The company has long stated its intent to examine the potential synergies of SRA737 with PARP inhibitors. PARP is a protein important for the DNA damage response (DDR), similar to Chk1 targeting by SRA737, although along a different axis. Therefore, the company has been interested in learning whether a combination strategy of inhibiting multiple DDR axes can improve clinical responses or expand the array of indications these drugs can effectively treat. There are currently three approved PARP inhibitors: Lynparza (olaparib, AstraZeneca), Rubraca (rucaparib, Clovis), and Zejula (niraparib, Tesaro/Janssen), all of which are approved to treat BRCA1/2 mutated cancers (ovarian and related cancers, Lynparza also approved for HER2-negative metastatic breast cancer).
The protein PARP is important for repairing DNA single-strand breaks and, when this pathway is inhibited, these single-strand breaks can become further damaged to double-strand breaks, which are repaired via the cell’s homologous recombination (HR) pathway. Cancer cells that are deficient in HR are particularly sensitive to PARP inhibition, which can occur with mutations to the BRCA1 and BRCA2 proteins (among others). The logic behind combining SRA737 with a PARPi is that Chk1 is also a key regulator of HR, and perhaps its inhibition can replicate the synthetic lethality seen in BRCA1/2 mutants.
A common resistance mechanism to PARP inhibitors is reversion of mutated BRCA1/2 to a functional form, restoring homologous recombination. SRA737 could potentially overcome BRCA1/2 reversion to restore activity following PARPi resistance because it inhibits HR through a different mechanism. The company has provided some preliminary in vitro evidence that SRA737 has a synergistic anti-cancer activity with PARP inhibition in PARP-resistant tissue cultures (Exhibit 8). The company will be presenting additional data on the activity of SRA737 in PARP resistant ovarian cancer at the AACR meeting in April 2018.
Exhibit 8: Synergistic activity of SRA737 and PARP inhibition in vitro
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Source: Sierra Oncology. Note: Shows stained PEO1-PR cells, from HGSOC, which developed PARP inhibitor resistance, units in µM.
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The company announced that it has entered into an agreement with Janssen to provide Zejula for an upcoming Phase Ib/II combination study with SRA737 in patients with mCRPC. We expect the trial to be of a similar dose ranging/expansion format to the current ongoing Phase I/II trials and to examine an array of tumor mutations. It is expected to initiate in Q418.